The study reported describes a combination of recombinant human bone morphogenetic protein-2 (rhBMP-2) and collagen (C) to regenerate bone. Unilateral critical-sized defects (CSDs) were prepared in radii of 32 skeletally mature New Zealand white rabbits. Rabbits were divided evenly among four treatments: autograft, absorbable C (Helistatt), 35 mg of rhBMP-2 combined with absorbable C (rhBMP-2/C), and untreated CSDs. The two euthanasia periods were 4 and 8 weeks. Radiographs were taken the day of surgery, every 2 weeks, and at term and the percent of radiopacity was measured. Data analysis revealed a time-dependent increase in the percent radiopacity with rhBMP-2/C. Histological examination revealed the rhBMP-2/C treatment regenerated osseous contour by 8 weeks. According to quantitative histomorphometry, the CSD and C groups had significantly less new bone than either autograft or rhBMP-2/C (p ¡ 0.05). The results suggest that rhBMP-2/C could be an effective therapy to restore segmental bone defects.
Hollinger, Jeffrey O.; Schmitt, John M.; Buck, David C.; Shannon, Robert; Joh, Seong-Pil; Zegzula, H Daniel; and Wozney, John, "Recombinant Human Bone Morphogenetic Protein-2 and Collagen for Bone Regeneration" (1998). Faculty Publications - Department of Biology and Chemistry. Paper 48.