A Simple Bioluminescent Method for Measuring D-Amino Acid Oxidase Activity

Authors

T. Spencer Bailey
Micah T. Donor, George Fox UniversityFollow
Sean P. Naughton
Michael D. Pluth

Document Type

Article

Publication Date

2015

Abstract

D-Amino acid oxidase (DAO) plays important roles in regulating D-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from D-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity based on the highly selective condensation of D-Cys with 6-hydroxy-2-cyanobenzothiazole (CBT-OH) to form D-luciferin.

Comments

Originally published in Chemical Communications, (2015), 51, 5425-5428.

https://doi.org/10.1039/C4CC08145E

Recommended Citation

Bailey, T. Spencer; Donor, Micah T.; Naughton, Sean P.; and Pluth, Michael D., "A Simple Bioluminescent Method for Measuring D-Amino Acid Oxidase Activity" (2015). Faculty Publications - Department of Biological & Molecular Science. 169.
https://digitalcommons.georgefox.edu/bio_fac/169