Eye lens β-crystallins are predicted by native ion mobility-mass spectrometry and computations to form compact higher-ordered heterooligomers

Authors

Amber D. Rolland
Takumi Takata
Micah T. Donor, George Fox UniversityFollow
Kirsten J. Lampi
James S. Prell

Document Type

Article

Publication Date

2023

Abstract

Eye lens crystallin proteins maintain the refractive properties of the lens but are not replaced after denucleation. Rolland et al. use native ion mobility-mass spectrometry, kinetics experiments, and computations to reveal that b-crystallins form heterodimers. These likely assemble into compact heterooligomers that enable the very high protein concentrations found in lens tissue.

Recommended Citation

Rolland AD, Takata T, Donor MT, Lampi KJ, Prell JS. Eye lens β-crystallins are predicted by native ion mobility-mass spectrometry and computations to form compact higher-ordered heterooligomers. 2023 Sep 7;31(9):1052-1064.e3. doi: 10.1016/j.str.2023.06.013.